Journal of Radiation and Cancer Research

TECHNICAL REPORT
Year
: 2017  |  Volume : 8  |  Issue : 1  |  Page : 82--86

Modified comet assays for the detection of cyclobutane pyrimidine dimers and oxidative base damages


Ganesan Muthusamy, Agilan Balupillai, Kanimozhi Govindasamy, Karthikeyan Ramasamy, Veeramani Kandan Ponniresan, Illiyas Magbool Malla, Rajendra Prasad Nagarajan 
 Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, Tamil Nadu, India

Correspondence Address:
Rajendra Prasad Nagarajan
Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, Tamil Nadu
India

The comet assay (also known as single-cell gel electrophoresis) is a technique for the detection of DNA damage at the level of the individual cell. It is a versatile, relatively simple to perform and sensitive method. Although most investigations make use of its ability to measure DNA single-strand breaks, modifications to the method allow detection of cyclobutane pyrimidine dimers (CPDs), crosslinks, base damage, and apoptotic nuclei. Many investigators also interested in examining the DNA damage as a function of time after exposure to a known genotoxic agent. Here, we present a procedure of comet assay for the detection of DNA strand breaks, base damages, and CPDs that can be used to measure DNA damage during toxicity, oxidative stress, and ultraviolet radiation exposure and it can be applied in human toxicological biomonitoring scenarios.


How to cite this article:
Muthusamy G, Balupillai A, Govindasamy K, Ramasamy K, Ponniresan VK, Malla IM, Nagarajan RP. Modified comet assays for the detection of cyclobutane pyrimidine dimers and oxidative base damages.J Radiat Cancer Res 2017;8:82-86


How to cite this URL:
Muthusamy G, Balupillai A, Govindasamy K, Ramasamy K, Ponniresan VK, Malla IM, Nagarajan RP. Modified comet assays for the detection of cyclobutane pyrimidine dimers and oxidative base damages. J Radiat Cancer Res [serial online] 2017 [cited 2021 Jan 27 ];8:82-86
Available from: https://www.journalrcr.org/article.asp?issn=2588-9273;year=2017;volume=8;issue=1;spage=82;epage=86;aulast=Muthusamy;type=0