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  Indian J Med Microbiol
 

Figure 5: The investigation of the mechanism of cytotoxicity induced by sodium salt of SCR7-pyrazine in cancer cells. (a) The determination of intracellular ROS production in MCF7 cells following treatment with sodium salt of SCR7-pyrazine. Cells treated with sodium salt of SCR7-pyrazine (100 μM) for different time periods (5, 10, 30, and 60 min) were used for assessing the formation of intracellular ROS by flow cytometry. H2O2-treated cells were used as positive control, while cells alone served as negative control. 1X phosphate buffered saline treated cells were used as vehicle control. Cell population showing reactive oxygen species was shown along with standard error mean (n = 2). (b) Evaluation of the effect of sodium salt of SCR7-pyrazine on mitochondrial membrane potential. MCF7 cells were treated with increasing concentrations of sodium salt of SCR7-pyrazine (0, 10, 50, 100, and 250 μM) for 48 h and alteration in mitochondrial membrane potential was assayed using JC-1 staining by flow cytometry. Bar diagram shows a spectral shift from red to green upon treatment. The ratio of red to green fluorescence is represented as bar diagram

Figure 5: The investigation of the mechanism of cytotoxicity induced by sodium salt of SCR7-pyrazine in cancer cells. (a) The determination of intracellular ROS production in MCF7 cells following treatment with sodium salt of SCR7-pyrazine. Cells treated with sodium salt of SCR7-pyrazine (100 μM) for different time periods (5, 10, 30, and 60 min) were used for assessing the formation of intracellular ROS by flow cytometry. H<sub>2</sub>O<sub>2</sub>-treated cells were used as positive control, while cells alone served as negative control. 1X phosphate buffered saline treated cells were used as vehicle control. Cell population showing reactive oxygen species was shown along with standard error mean (<i>n</i> = 2). (b) Evaluation of the effect of sodium salt of SCR7-pyrazine on mitochondrial membrane potential. MCF7 cells were treated with increasing concentrations of sodium salt of SCR7-pyrazine (0, 10, 50, 100, and 250 μM) for 48 h and alteration in mitochondrial membrane potential was assayed using JC-1 staining by flow cytometry. Bar diagram shows a spectral shift from red to green upon treatment. The ratio of red to green fluorescence is represented as bar diagram