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  Indian J Med Microbiol
 

Figure 4: Evaluation of effect of sodium salt of SCR7-pyrazine on cell cycle progression and viability of different cancer cells. (a) Effect of sodium salt of SCR7-pyrazine on cell viability and proliferation was assessed by trypan blue assay. REH, Nalm6, T47D, MCF7, HeLa, Molt4, CEM, HEK293T cells were cultured (0.5 × 105 cells/ml) in the presence of sodium salt of SCR7-pyrazine (0, 10, 50, 100 and 250 μM), harvested after 48 h and used for analysis. 1X phosphate buffered saline treated cells were used as vehicle control. Data presented are based on three or more independent experiments, and error bars represent standard error of mean. In all panels, P value represents * P < 0.05; **P < 0.01; ***P < 0.001. (b) Table showing IC50 was calculated post 48 h of treatment using GraphPad Prism 6.0 (Graph Pad, California, San Diago, USA). (c) Sodium salt of SCR7-pyrazine (0, 10, 50, 100, and 250 μM) was added to MCF7 cells and harvested post 48 h of treatment. The cells were stained with propidium iodide and DNA content was quantified by flow cytometry. 1X phosphate buffered saline treated cells served as the vehicle control. For each sample minimum of 10,000 cells were acquired. In each case, M1 represents sub G1 phase, M2 is G1 phase, M3 is S phase, and M4 represents G2/M phase. X-axis corresponds to PI-stained cells. (d) Bar diagram showing the percentage of cells in the sub G1, G1, S, and G2/M phase of the cell cycle obtained after FACS analyses based on three independent repeats

Figure 4: Evaluation of effect of sodium salt of SCR7-pyrazine on cell cycle progression and viability of different cancer cells. (a) Effect of sodium salt of SCR7-pyrazine on cell viability and proliferation was assessed by trypan blue assay. REH, Nalm6, T47D, MCF7, HeLa, Molt4, CEM, HEK293T cells were cultured (0.5 × 10<sup>5</sup> cells/ml) in the presence of sodium salt of SCR7-pyrazine (0, 10, 50, 100 and 250 μM), harvested after 48 h and used for analysis. 1X phosphate buffered saline treated cells were used as vehicle control. Data presented are based on three or more independent experiments, and error bars represent standard error of mean. In all panels, <i>P</i> value represents * <i>P</i> < 0.05; **<i>P</i> < 0.01; ***<i>P</i> < 0.001. (b) Table showing IC<sub>50</sub> was calculated post 48 h of treatment using GraphPad Prism 6.0 (Graph Pad, California, San Diago, USA). (c) Sodium salt of SCR7-pyrazine (0, 10, 50, 100, and 250 μM) was added to MCF7 cells and harvested post 48 h of treatment. The cells were stained with propidium iodide and DNA content was quantified by flow cytometry. 1X phosphate buffered saline treated cells served as the vehicle control. For each sample minimum of 10,000 cells were acquired. In each case, M1 represents sub G1 phase, M2 is G1 phase, M3 is S phase, and M4 represents G2/M phase. X-axis corresponds to PI-stained cells. (d) Bar diagram showing the percentage of cells in the sub G1, G1, S, and G2/M phase of the cell cycle obtained after FACS analyses based on three independent repeats