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  Indian J Med Microbiol
 

Figure 3: Effect of the sodium salt of SCR7-pyrazine on joining by Ligase IV/XRCC4, Ligase IIIα/XRCC1, Ligase I and T4 DNA Ligase. (a) The evaluation of the inhibitory effect of increasing concentrations of sodium salt of SCR7-pyrazine (0, 10, 50, 100, 200, 500, and 1000 μM) on Ligase IV-mediated joining of DNA containing 5'-5' compatible ends. DNA Ligase IV/XRCC4 complex was purified and used for the study. DNA Ligase IV-mediated joining in the presence of 1X phosphate buffered saline is shown (lane 2, 3). (b and c) Evaluation of the inhibitory effect of increasing concentrations of sodium salt of SCR7-pyrazine (0, 50, 100, 200, 500, and 1000 μM) on joining of nicked duplex DNA substrate, catalyzed by either purified DNA Ligase III/XRCC1 (b) or DNA Ligase I. (c and d) The analysis of inhibitory effect of increasing concentrations of sodium salt of SCR7-pyrazine (0, 50, 100, 200, 500, and 1000 μM) on joining of DNA substrate containing 5'-5' compatible ends catalyzed by T4 DNA Ligase. In all panels, inhibition due to the addition of sodium salt of SCR7-pyrazine to respective DNA Ligase mediated joining is quantified and presented. Lane 1 is DNA substrate alone (no protein added). In panels, (a-d) control joining reaction in the absence of inhibitor is shown (lane 2). 'M' is 50 bp ladder. The joined products are indicated in all panels. Error bars represent standard error of mean and P value indicates, *P < 0.05; **P < 0.01; ***P < 0.001

Figure 3: Effect of the sodium salt of SCR7-pyrazine on joining by Ligase IV/XRCC4, Ligase IIIα/XRCC1, Ligase I and T4 DNA Ligase. (a) The evaluation of the inhibitory effect of increasing concentrations of sodium salt of SCR7-pyrazine (0, 10, 50, 100, 200, 500, and 1000 μM) on Ligase IV-mediated joining of DNA containing 5'-5' compatible ends. DNA Ligase IV/XRCC4 complex was purified and used for the study. DNA Ligase IV-mediated joining in the presence of 1X phosphate buffered saline is shown (lane 2, 3). (b and c) Evaluation of the inhibitory effect of increasing concentrations of sodium salt of SCR7-pyrazine (0, 50, 100, 200, 500, and 1000 μM) on joining of nicked duplex DNA substrate, catalyzed by either purified DNA Ligase III/XRCC1 (b) or DNA Ligase I. (c and d) The analysis of inhibitory effect of increasing concentrations of sodium salt of SCR7-pyrazine (0, 50, 100, 200, 500, and 1000 μM) on joining of DNA substrate containing 5'-5' compatible ends catalyzed by T4 DNA Ligase. In all panels, inhibition due to the addition of sodium salt of SCR7-pyrazine to respective DNA Ligase mediated joining is quantified and presented. Lane 1 is DNA substrate alone (no protein added). In panels, (a-d) control joining reaction in the absence of inhibitor is shown (lane 2). 'M' is 50 bp ladder. The joined products are indicated in all panels. Error bars represent standard error of mean and <i>P</i> value indicates, *<i>P</i> < 0.05; **<i>P</i> < 0.01; ***<i>P</i> < 0.001