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  Indian J Med Microbiol
 

Figure 2: The effect of sodium salt of SCR7-pyrazine on DNA end joining catalyzed by rat testicular cell-free extracts. (a) Schematic representation of nonhomologous end-joining assay in the presence of inhibitor. (b) Effect of increasing concentrations of the sodium salt of SCR7-pyrazine (0, 50, 100, 200, 500, and 1000 μM) on end joining of double-strand breaks bearing 5'-5' compatible ends, catalyzed by cell-free extracts from rat testes (25°C for 60 min). (c) Bar diagram showing quantification of the inhibition of joining of 5'-5' compatible end substrates by sodium salt of SCR7-pyrazine, based on the three independent experiments. (d) Effect of increasing concentrations of sodium salt of SCR7-pyrazine (0, 50, 100, 200, 500, and 1000 μM) on double-strand break joining of 5'-3' or 5'-5' noncompatible end structures, catalyzed by rat testicular cell-free extracts (25°C, 60 min). (e) Bar diagram representing quantification of inhibition of joining of double-strand breaks containing either 5'-3' (upper) or 5'-5' (lower) noncompatible termini in the presence of sodium salt of SCR7-pyrazine on a minimum of two independent experiments. In all panels, the concentrations of sodium salt of SCR7-pyrazine used are indicated. Joining products are indicated. 'M' is 50 bp ladder. No protein control (lane 1, 8) and joining in the presence of vehicle control (1X phosphate buffered saline, lanes 2, 9) is also shown. In all panels, error bars represent standard error of mean and P value indicates, *P < 0.05; **P < 0.01; NS: Non-significant

Figure 2: The effect of sodium salt of SCR7-pyrazine on DNA end joining catalyzed by rat testicular cell-free extracts. (a) Schematic representation of nonhomologous end-joining assay in the presence of inhibitor. (b) Effect of increasing concentrations of the sodium salt of SCR7-pyrazine (0, 50, 100, 200, 500, and 1000 μM) on end joining of double-strand breaks bearing 5'-5' compatible ends, catalyzed by cell-free extracts from rat testes (25°C for 60 min). (c) Bar diagram showing quantification of the inhibition of joining of 5'-5' compatible end substrates by sodium salt of SCR7-pyrazine, based on the three independent experiments. (d) Effect of increasing concentrations of sodium salt of SCR7-pyrazine (0, 50, 100, 200, 500, and 1000 μM) on double-strand break joining of 5'-3' or 5'-5' noncompatible end structures, catalyzed by rat testicular cell-free extracts (25°C, 60 min). (e) Bar diagram representing quantification of inhibition of joining of double-strand breaks containing either 5'-3' (upper) or 5'-5' (lower) noncompatible termini in the presence of sodium salt of SCR7-pyrazine on a minimum of two independent experiments. In all panels, the concentrations of sodium salt of SCR7-pyrazine used are indicated. Joining products are indicated. 'M' is 50 bp ladder. No protein control (lane 1, 8) and joining in the presence of vehicle control (1X phosphate buffered saline, lanes 2, 9) is also shown. In all panels, error bars represent standard error of mean and <i>P</i> value indicates, *<i>P</i> < 0.05; **<i>P</i> < 0.01; NS: Non-significant