• Users Online: 247
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
TECHNICAL REPORT
Year : 2017  |  Volume : 8  |  Issue : 1  |  Page : 82-86

Modified comet assays for the detection of cyclobutane pyrimidine dimers and oxidative base damages


Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, Tamil Nadu, India

Correspondence Address:
Rajendra Prasad Nagarajan
Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, Tamil Nadu
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-0168.199312

Rights and Permissions

The comet assay (also known as single-cell gel electrophoresis) is a technique for the detection of DNA damage at the level of the individual cell. It is a versatile, relatively simple to perform and sensitive method. Although most investigations make use of its ability to measure DNA single-strand breaks, modifications to the method allow detection of cyclobutane pyrimidine dimers (CPDs), crosslinks, base damage, and apoptotic nuclei. Many investigators also interested in examining the DNA damage as a function of time after exposure to a known genotoxic agent. Here, we present a procedure of comet assay for the detection of DNA strand breaks, base damages, and CPDs that can be used to measure DNA damage during toxicity, oxidative stress, and ultraviolet radiation exposure and it can be applied in human toxicological biomonitoring scenarios.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1648    
    Printed95    
    Emailed0    
    PDF Downloaded239    
    Comments [Add]    
    Cited by others 7    

Recommend this journal